• Crowe Lab 2009


  • Latest News
  • Recent Publications
  • Dr. Crowe will present his lecture, "Next Generation Vaccines" on Thursday, May 22, at 4 pm in 208 Light Hall as a part of Vanderbilt's Discovery Lecture series. This series seeks to respond to the requests of the Biomedical Advisory Board in presenting discussion-stimulating topics in research. Dr. Borden Lacy and Dr. Simon Mallal will also present findings that showcase Vanderbilt University's position on the cutting-edge of medical research; their lectures are respectively entitled "Structure and Function of Clostridium difficile Toxins A and B" and "Personalized Immunology." Learn more about the Discovery Lecture series.
  • While the H5N1 avian influenza virus does not seem to transmit from person to person, scientists in the Netherlands and at the University of Wisconsin have found that the deadly virus can acquire aerosol transmissibility when passed intentionally in the laboratory from ferret to ferret. This finding created many fears in the scientific community of bioterrorist activity through the use of this information about the H5N1 avian influenza virus. Vanderbilt research, however, shows that human antibodies to the natural strain of H5N1 are able to kill the potentially more harmful laboratory-created strain of the H5N1 avian flu virus. Read more in the Reporter.
  • On June 28th, Dr. Crowe was interviewed by the Voice of Russia about a gold particle vaccine to prevent respiratory syncytial virus (RSV). In this new method, tiny gold particles mimic a virus and carry specific proteins to immune cells. In addition to using a technology of coating or embedding a particle, the Crowe Lab is simulating the shape of the virus by using techniques to allow the gold to mirror the rod-like structure of RSV. This gold particle vaccine for RSV could bring about a "possible gold rush" in medicine, where gold particles would serve as a platform technology for research and vaccine development for other viruses, and possibly even for treatment of bacterial infections or cancer. Future research may also analyze the structure of gold to discover other materials that could be used in this method in place of gold. Click here to hear Dr. Crowe's interview.
  • James Crowe Jr., MD and Alexander Bukreyev, PhD from the University of Texas Medical Branch at Galveston have received a $4.4 million grant from the U.S. Department of Defense to explore and develop prevention and treatment options for Ebola and Marburg viruses. These viruses are known for their high death rates, which are around 80 percent, and for the intense course of their illnesses, which includes bleeding and multi-organ failure. The viruses can be spread to humans from an animal vector and are spread from person to person through blood contact. Crowe and his team will isolate antibodies from blood cells of survivors of Marburg or Ebola virus hemorrhagic fevers. Crowe and Bukreyev have received more than 1,000 blood cell samples from 30 Ugandan survivors of Ebola and from a Colorado woman who contracted Marburg viral illness while visiting Uganda in 2008. The lab is still awaiting samples from the Congo and from Gabon so that all known Ebola and Marburg viral strains are covered. The goal of this project is to test the effectiveness of human antibodies as both preventive and treatment measures in animal models. Read more in the Reporter.
  • On January 11, 2013, Crowe Lab Manager Frances Smith-House was named the recipient of the Edward E. Price Jr. Award for Excellence in Basic Research, one of three 2012 Research Staff Awards at Vanderbilt University Medical Center. Fran has served as an innovative and fearless leader in the Crowe Lab. According to Dr. Crowe's comments in the VUMC Reporter, Fran's expertise has been a huge contribution to the isolation of immune cells from survivors of the 1918 influenza pandemic, generating "some of the most unique human monoclonal antibodies ever described." The VVC is excited to celebrate Fran's award and is grateful for her contributions to the VVC. Read more in the Reporter.
  • Dr. Crowe was honored with the "Mentor of the Year" award for 2011 at the Sixth Annual VUMC Postdoctoral Research and Shared Resources Symposium held on May 2nd, 2012.
  • Wen X, Krause JC, Leser JC, Lamb RA, Williams JV, Crowe JE Jr, Jardetsky TS. The structure of the human metapneumovirus fusion protein in complex with a human neutralizing antibody. In press, Nature Structural and Molecular Biology 2012.
  • Lindquist ME, Mainou B, Dermody T, Crowe JE Jr. Activation of protein kinase R is required for induction of stress granules by respiratory syncytial virus but dispensable for viral replication. Virology 2011; 413:103–110. PMID: 21377708.
  • Shaikh FY, Cox RG, Lifland AW, Hotard AL, Williams JV, Moore ML, Santangelo PJ, Crowe JE Jr. A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. mBio 2012; 3(1):e00270-11. doi: 10.1128.
  • Smith SA, Zhou Y, Olivarez NP, Broadwater AH, de Silva AM, Crowe. Persistence of circulating B memory cell clones with potential for dengue virus disease enhancement for decades following infection. Journal of Virology 2011 Dec 14. [Epub ahead of print] PMID: 22171265.
  • Engelhardt BG, Griffith ML, Crowe JE Jr, Savani BN, Kassim AA, Lu P, Weitkamp JH, Moore D, Yoder S, Rock MT, Jagasia SM, Jagasia M. Predicting post-transplant diabetes mellitus by regulatory T cell phenotype: Implications for metabolic intervention to modulate alloreactivity. Blood 2012 Jan 19. [Epub ahead of print] PMID: 22262764.
  • Zhang GL, Ansari HR, Bradley P, Cawley GC, Hertz T, Hu X, Huang JC, Jojic N, Kim Y, Kohlbacher O, Lund O, Lundegaard C, Magaret CA, Nielsen M, Papadopoulos H, Raghava GPS, Tal V-S, Xue L, Yanover C, Zhang H, Zhu S, Rock MT, Crowe JE Jr, Panayiotou C, Polycarpou MM, Duch W, Brusic V. Machine learning competition in immunology – Prediction of HLA class I molecules. Journal of Immunological Methods 2011; 374:1-4.
  • Rock MT, McKinney BA, Yoder SM, Prudom CE, Wright DW, Crowe JE Jr. Identification of potential human respiratory syncytial virus and metapneumovirus T cell epitopes using computational prediction and MHC binding assays. Journal of Immunological Methods 2011; 374:13-17. NIHMS323653.
  • Krause JC, Ekiert DC, Tumpey TM, Smith PB, Wilson IA, Crowe JE Jr. An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody. mBio 2011; 2(1):e00345-10. doi:10.1128/mBio.00345-10. PMCID: 3037006.
  • Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Briney BS, Smith SA, Basler CF, Crowe JE Jr. Epitope-specific human antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergence. Journal of Immunology 2011; 187:3704-11. PMC3178754.
  • Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Basler CF, Crowe JE Jr. A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of influenza H1N1 virus hemagglutinin. Journal of Virology 2011; 85:10905-08. PMID: 21849447.
  • Joyner AS, Willis JR, Crowe JE Jr, Aiken C. Maturation-induced cloaking of neutralization epitopes on HIV-1 particles. PLoS Pathogens 2011; 7(9): e1002234. doi:10.1371/journal.ppat.1002234
  • Dochow M, Krumm SA, Crowe JE Jr, Moore ML, Plemper RK. Independent structural domains in paramyxovirus polymerase proteins. Journal of Biological Chemistry 2012, 10.1074/jbc.M111.325258, Jan 3. [Epub ahead of print] PMID: 22215662