• Crowe Lab 2009


  • Latest News
  • Recent Publications
  • Dr. Crowe was honored with the "Mentor of the Year" award for 2011 at the Sixth Annual VUMC Postdoctoral Research and Shared Resources Symposium held on 2nd May, 2012.
  • Scientists at Emory University recently published an interesting paper on the functional domains of paramyxovirus proteins. These viruses include major human pathogens, such as measles virus and RSV. The work, entitled “Independent Structural Domains in Paramyxovirus Polymerase Proteins” was conducted in collaboration with Dr. Crowe and was published in the Journal of Biological Chemistry.
  • Iuliia Gilchuk, PhD recently joined the Vaccine Center as postdoctoral research scientist in Crowe laboratory. Dr. Gilchuk will study human neutralizing antibodies induced by prior natural smallpox or monkeypox infection or immunization with the smallpox vaccine, known as vaccinia virus. She has extensive scientific experience in molecular antibody studies and in protein expression. The studies are supported by a major NIH contract for human B cell epitope mapping.
  • Andre Branchizio has recently joined the Vaccine Center as scientific information technology specialist. Andre brings programming and other computational skills to to the VVC and Crowe laboratory. He will focus on developing databases and analytical tools for ultra large-scale studies of human immune repertoires using high-throughput sequence analysis.
  • Scott Smith, MD, PhD, an Instructor in the Department of Medicine (Infectious Diseases) and member of the Crowe laboratory, authored a paper on human antibodies to dengue viruses. The paper has been published in the Journal of Virology. The paper is entitled “Persistence of circulating B memory cell clones with potential for dengue virus disease enhancement for decades following infection.” Dengue is a mosquito borne virus that affects a large portion of the world’s population and has also recently spread to Florida in the U.S. The paper reveals that patients maintain B cells in their circulation specifying antibodies that enhance the disease for many years following infection. The work also identified some rare B cells that principally neutralize the virus without enhancing other serotypes. Understanding the nature of these antibodies is of interest for rational design of dengue vaccines.
  • Fyza Shaikh, a student in the Medical Scientist (MD/PhD) training program, authored an important paper about assembly and budding of respiratory syncytial virus (RSV) that will be published in the journal mBio. RSV is the main cause of wheezing and pneumonia in infants and children. The paper is entitled “A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles”. The work reveals the role of the cytoplasmic tail of the viral fusion protein in assembly and buddy, and made the remarkable observation that a single amino acid in the tail controls the process of formation of virus.
  • Bryan Briney, a PhD candidate student in the Department of Pathology, Microbiology and Immunology Department and the Crowe laboratory, was selected for a travel award to the Keystone Conference on HIV Vaccines (X5) in Keystone, CO in March 2012. He will present work on the preimmune repertoire of human antibodies that bind to HIV. Seroepidemiology studies show that patients infected with HIV typically do not develop broad or potent neutralizing antibodies to HIV for many months or several years after infection. One hypothesis is that the early response to HIV is dominated by poor quality antibodies. In order to explore this idea further, Bryan isolated antibodies from healthy subjects who had never been infected with HIV. Surprisingly, healthy subjects have a significant number of B cells in the blood with the potential to bind HIV. Molecular characterization of these clones sheds light on the antibodies that likely would be the “first responders” to HIV infection.
  • Valentine Chukwuma, a PhD candidate graduate student in the Department of Pathology, Microbiology and Immunology Department and the Crowe laboratory, recently passed his PhD thesis proposal defense (qualifying exam). He will study the molecular and genetic basis for development of neutralizing breadth in HIV infected subjects in a longitudinal patient cohort in Nashville. The studies, in collaboration with Spyros Kalams, MD of Vanderbilt and in collaboration with scientists led by Nancy Haigwood, PhD of Oregon Health Sciences University, are supported by an NIH HIV Vaccine Research and Design (HIVRAD) award.
  • Gopal Sapparapu, PhD won the Best Poster award in January 2012 at PepTalk 2012 in San Diego, CA. Cambridge Healthtech Institute's PepTalk: The Protein Science Week is the premier meeting event in the protein industry. His presentation entitled “Intracellular delivery of an anti-rotavirus bispecific diabody” detailed his work on a new antibody technology.
  • Jordan Willis, a PhD candidate student in the Program for Chemical and Physical Biology and the Crowe laboratory, was recently invited to present new research findings at the Keystone Conference on HIV Vaccines (X5) in Keystone, CO in March 2012. His presentation will review new methods for computational experiments that define the structural basis for neutralization of HIV by human monoclonal antibodies.
  • Wen X, Krause JC, Leser JC, Lamb RA, Williams JV, Crowe JE Jr, Jardetsky TS. The structure of the human metapneumovirus fusion protein in complex with a human neutralizing antibody. In press, Nature Structural and Molecular Biology 2012.
  • Lindquist ME, Mainou B, Dermody T, Crowe JE Jr. Activation of protein kinase R is required for induction of stress granules by respiratory syncytial virus but dispensable for viral replication. Virology 2011; 413:103–110. PMID: 21377708.
  • Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Briney BS, Smith SA, Basler CF, Crowe JE Jr. Epitope-specific human antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergence. Journal of Immunology 2011; 187:3704-11. PMC3178754.
  • Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Basler CF, Crowe JE Jr. A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of influenza H1N1 virus hemagglutinin. Journal of Virology 2011; 85:10905-08. PMID: 21849447.
  • Joyner AS, Willis JR, Crowe JE Jr, Aiken C. Maturation-induced cloaking of neutralization epitopes on HIV-1 particles. PLoS Pathogens 2011; 7(9): e1002234. doi:10.1371/journal.ppat.1002234
  • Dochow M, Krumm SA, Crowe JE Jr, Moore ML, Plemper RK. Independent structural domains in paramyxovirus polymerase proteins. Journal of Biological Chemistry 2012, 10.1074/jbc.M111.325258, Jan 3. [Epub ahead of print] PMID: 22215662
  • Smith SA, Zhou Y, Olivarez NP, Broadwater AH, de Silva AM, Crowe. Persistence of circulating B memory cell clones with potential for dengue virus disease enhancement for decades following infection. Journal of Virology 2011 Dec 14. [Epub ahead of print] PMID: 22171265.
  • Shaikh FY, Cox RG, Lifland AW, Hotard AL, Williams JV, Moore ML, Santangelo PJ, Crowe JE Jr. A critical phenylalanine residue in the respiratory syncytial virus fusion protein cytoplasmic tail mediates assembly of internal viral proteins into viral filaments and particles. mBio 2012; 3(1):e00270-11. doi: 10.1128.
  • Krause JC, Ekiert DC, Tumpey TM, Smith PB, Wilson IA, Crowe JE Jr. An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody. mBio 2011; 2(1):e00345-10. doi:10.1128/mBio.00345-10. PMCID: 3037006.
  • Engelhardt BG, Griffith ML, Crowe JE Jr, Savani BN, Kassim AA, Lu P, Weitkamp JH, Moore D, Yoder S, Rock MT, Jagasia SM, Jagasia M. Predicting post-transplant diabetes mellitus by regulatory T cell phenotype: Implications for metabolic intervention to modulate alloreactivity. Blood 2012 Jan 19. [Epub ahead of print] PMID: 22262764.
  • Zhang GL, Ansari HR, Bradley P, Cawley GC, Hertz T, Hu X, Huang JC, Jojic N, Kim Y, Kohlbacher O, Lund O, Lundegaard C, Magaret CA, Nielsen M, Papadopoulos H, Raghava GPS, Tal V-S, Xue L, Yanover C, Zhang H, Zhu S, Rock MT, Crowe JE Jr, Panayiotou C, Polycarpou MM, Duch W, Brusic V. Machine learning competition in immunology – Prediction of HLA class I molecules. Journal of Immunological Methods 2011; 374:1-4.
  • Rock MT, McKinney BA, Yoder SM, Prudom CE, Wright DW, Crowe JE Jr. Identification of potential human respiratory syncytial virus and metapneumovirus T cell epitopes using computational prediction and MHC binding assays. Journal of Immunological Methods 2011; 374:13-17. NIHMS323653.